Light Microscopy
Compound Microscope
The EM Unit has an Olympus BHS microscope equipped with a Model BH2-RFL
attachment for fluorescence microscopy.
- When used with transmitted light it can be operated in brightfield,
phase contrast or differential interference contrast (Nomarski) modes
- Illumination from the mercury burner allows bright, clear fluorescent
image formation against a dark background
- Magnification ranges from x40 to x1000
- The microscope is fitted with the new Leica DC300 F digital camera
for the acquisition of digital images with dimensions of up to 1950
x 1545 pixels.

Micrographs taken with this equipment.

Transverse section through a leaf of Phebalium glandulosum,
stained with Toluidine blue.

Transverse section of unfolding leaf within coleoptile of Hordeum
vulgare, stained with calcofluor.
Dissecting Microscopes
The microscopy facility now also has a Leica MZ FLIII fluorescent dissecting
microscope for the imaging of fluorescently labelled whole cells and
tissues, with magnifications up to 400x.

We also have a Leica MZ6 dissecting microscope with vertical illumination
and flexible light guides for routine specimen preparation.

|