Recent Published Work by the ScUM group.             2002 – present

Dr Rick Wetherbee (Head of ScUM)

Book Chapters (refereed)

Hildebrand, M. and Wetherbee, R. (2002) Components and Control of Silicification in Diatoms. In Silicon Biomineralization (Ed. W.E.G. Mueller), Springer-Verlag, Berlin, pp.12-57.

Wetherbee, R., Crawford, S.A. and Mulvaney, P., (2004) The nanostructure and development of diatom biosilica. In Biomineralization (Ed. E. Baeuerlein), Robert Wiley Press, pp. 177-193.

Chiovitti, A., Dugdale, T.M., and Wetherbee, R. (2006) Diatom adhesives: molecular and mechanical properties. In Biological Adhesives (Eds. A.C. Smith and J.A. Callow), Springer-Verlag, Heidelberg, Germany, (in press).

Journal Articles (all in international, refereed journals)

Callow, M.E., Crawford, S.A., Wetherbee, R., Taylor, K., Finlay, J.A. and Callow, J.A. (2001) Brefeldin A affects adhesion of zoospores of the green alga Enteromorpha. J. Exp. Bot. 52:1409-1415.

Crawford, S.A., Higgins, M.J., Mulvaney, P., and Wetherbee, R. (2001) The nanostructure of the diatom frustule as revealed by atomic force and scanning electron microscopy. J. Phycol. 34: 555-573.

Schultz, T.F., Egerton-Warburton, L, Crawford, S.A. and Wetherbee, R. (2001) Identification of a 41 kDa protein embedded in the biosilica of scales and bristles isolated from Mallomonas splendens (Synurophyceae, Ochrophyta). Protist 152: 315-327.

Higgins, M.J., Crawford, S.A., Mulvaney, P. and Wetherbee, R. (2002) Characterization of the adhesive mucilages secreted by live diatom cells using atomic force microscopy. Protist 153:25-38.

*Apt, K.E., Zaslavkaia, L., Lippmeier, J. C., Lang, M., Kilian, O.,Wetherbee, R., Grossman, A.R. and Kroth, P.G. (2002) In vivo characterization of diatom multipartite plastid targeting signals. J. Cell Science 115:5061-4069.

Higgins, M.J., Sader, J.E., Mulvaney,P. and Wetherbee,R. (2003) Probing the surface of living diatoms with atomic force microscopy: the nanostructure and micromechanical properties of the mucilage layer. J. Phycol. 39:722-73

Chiovitti A., Higgins M.J., Harper R.E., Wetherbee R., and Bacic A. (2003). The complex polysaccharides of the raphid diatom Pinnularia viridis (Bacillariophyceae). J. Phycol. 39: 543-554.

Higgins, M.J., Molino, P., Mulvaney, P. and Wetherbee, R. (2003) Characterizing the structure and nanomechanical properties of the adhesive mucilage that mediates diatom-substratum adhesion and motility. J. Phycol.39:1181-1193.

Chiovitti A., Bacic A., Burke J., and Wetherbee R. (2003). Heterogeneous xylose-rich glycans are associated with extracellular glycoproteins from the biofouling diatom Craspedostauros australis (Bacillariophyceae). Eur. J. Phycol. 38: 351-360.

Chiovitti, A., Molino, P., Crawford, S.A., Teng, R., Spurck, T., and Wetherbee, R. (2004) Water-extractable glucans from diatoms are predominantly derived from intracellular chrysolaminaran and not extracellular polysaccharides. Eur. J. Phycol. 39: 117-128

Holland, R., Dugdale, T.M., Wetherbee, R., Brennan, A.B., Finlay, J.A, Callow, J.A., and Callow, M.E. (2004) Adhesion and Motility of Fouling Diatoms on a Silicone Elastomer Biofouling, 20 : 323 - 329

Dugdale, T.M., Dagastine, R., Chiovitti, A., Mulvaney, P., and Wetherbee, R. (2005) Single adhesive nanofibers from a live diatom have the signature fingerprint of modular proteins.
Biophysical J. 89:4252-4260.

Chiovitti, A., Harper, R., Willis, A., Bacic, A., Mulvaney, P., and Wetherbee, R. (2005) Variations in the substituted 3-linked mannans closely associated with silicified walls of diatoms. J. Phycol. 41: 1154-1161

Dugdale, T.M., Dagastine, R., Chiovitti, A. and Wetherbee, R. (2006) Diatom adhesive mucilage contains distinct supramolecular assemblies of a single modular protein. Biophysical J. (Biophys J BioFAST, published on-line January 27, 2006 as doi:10.1529/biophysj.105.079129 )

Dugdale, T.M., Willis, A. and Wetherbee, R. (2006) Adhesive modular proteins occur in the extracellular mucilage of the motile, pennate diatom Phaeodactylum tricornutum. Biophysical J. - Biophysical Letters. (published on-line on Biophys J bioFAST)

Other publications

*Wetherbee, R. (2002) "The Diatom Glasshouse", Science Perspectives. Science 298:547.

Dr Tony Chiovitti  (Research Fellow, group member)

Invited paper

*Armbrust, E.V., Allen, A., Saito, M.A., Bowler, C., Monstant, A., Apt, K.E., Lippmeier, J.C., Berges, J.A., Brzezinski, M.A., Demarest, M., Chiovitti, A., Davis, A., Hilldebrand, M., Thamatakoln, K., Palenik, B., Green, B.R., Orbornik, M., Chaal, B.K., Hadi, M., Lane, T.W., Jenkins, B., Kröger, N., Lau, W., Parker, M., Rynearson, T.A., Pazour, G.J., Valentin, K., Wilkerson, F.P., Schwartz, D., Zho, S., Rokshar, D., Martinez, D., Terry, A., Huang, K., Dehal, P., Goodstein, D.M., Medina, M., Chapman, J., Putnam, N., Larimer, F., Kapitonov, V.V., Jurka, J.  2004.  Insights into the ecological and evolutionary success of diatoms revealed by whole genome analysis of the marine centric diatom Thalassiosira pseudonana (tentative title).  Solicited by Science and overseen by Dr Virginia Armbrust (University of Washington, USA, e-mail: armbrust@ocean.washington.edu). 

Journal articles

Chiovitti, A., Bacic, A., Craik, D.J., Kraft, G.T., and Liao, M.-L.  2004.  A nearly idealized 6’-O-methylated ι-carrageenan from the Australian red alga Claviclonium ovatum (Acrotylaceae, Gigartinales).  Carbohydrate Research 339: 1459-1466.

*Chiovitti, A., Molino, P., Crawford, S.A., Teng, R., Spurck, T., and Wetherbee, R.  2004.  The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides.  EuropeanJournal of Phycology 39: 117-128. 

Chiovitti, A., McManus, L.J., Kraft, G.T., Bacic, A., and Liao, M.-L.  2004.  Extraction and characterization of agar from Australian Pterocladia lucida.  Journal of Applied Phycology 16: 41-48.

Saunders, G.W., Chiovitti, A., and Kraft, G.T.  2004.  Small-subunit rDNA sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta).  3.  Delineating the Gigartinales sensu stricto.  Canadian Journal of Botany 81: 43-74.

Hernández-Daranas, A., Kalverda, A.P., Chiovitti, A., and Homans, S.W.  2004.  Letter to the Editor: Backbone resonance assignment of the L-arabinose binding protein in complex with D-galactose.  Journal of Biomolecular NMR 28: 91-92. 

*Chiovitti, A., Higgins, M.J., Harper, R.E., Wetherbee, R., and Bacic, A.  2003.  The complex polysaccharides of the raphid diatom Pinnularia viridis (Bacillariophyceae).  Journal of Phycology 39: 543-554.

*Chiovitti, A., Bacic, A., Burke, J., and Wetherbee, R.  2003.  Heterogeneous xylose-rich glycans are associated with extracellular glycoproteins from the biofouling diatom Craspedostauros australis (Bacillariophyceae).  EuropeanJournal of Phycology 38: 351-360.

Watt, N.J., Chiovitti, A., Craik, D.J., and Kraft, G.T.  2003.  The cell wall galactans from Australian representatives of the genus Meristotheca (Solieriaceae, Rhodophyta).  Phycologia 42: 572-581. 

Chiovitti, A., Kraft, G.T., Bacic, A., Craik, D.J., and Liao, M.-L.  2001.  Chemistry, properties, and phylogenetic significance of the methylated carrageenans from red algae of the genus Areschougia (Areschougiaceae, Gigartinales, Rhodophyta).  Journal of Phycology 37: 1127-1137.

Chiovitti, A., Kraft, G.T., Bacic, A., Liao, M.-L.  2001.  Gelling polysaccharides from Australian seaweeds: research and potential.  Marine and Freshwater Research 52: 917-935.

Chiovitti, A., Bacic, A., Kraft, G.T., Craik, D.J., and Liao, M.-L.  1999.  Pyruvated carrageenans from Solieria robusta and its adelphoparasite Tikvahiella candida.  Hydrobiologia 398/399: 401-409.